Chimeric Phi29 DNA polymerase with helix-hairpin-helix motifs exhibits enhanced salt tolerance and replication efficiency
Phi29 DNA polymerase (Phi29 Pol) has been efficiently utilized in DNA nanoball-based sequencing, real-time DNA sequencing from single polymerase molecules and nanopore sequencing using the sequencing by synthesis (SBS) technique. Amongst these, polymerase-assisted nanopore sequencing expertise analyses nucleotide sequences as a operate of modifications in electrical present. This ionic, current-based sequencing expertise requires polymerases to carry out replication at excessive salt concentrations, for instance 0.three M KCl . Nonetheless, the salt tolerance of wild-type Phi29 Pol is comparatively low.
Right here, we fused helix-hairpin-helix (HhH)2 domains E-L (eight repeats in whole) of topoisomerase V (Topo V) from the hyperthermophile Methanopyrus kandleri to the Phi29 Pol COOH terminus, designated Phi29EL DNA polymerase (Phi29EL Pol). Area fusion elevated the general enzyme replication effectivity by fourfold. Phi29EL Pol catalysed rolling circle replication in a broader vary of salt concentrations than did Phi29 Pol, extending the KCl focus vary for exercise as much as 0.three M. As well as, the mutation of Glu375 to Ser or Gln elevated Phi29EL Pol exercise within the presence of KCl. On this work, we produced a salt-tolerant Phi29 Pol by-product by the use of (HhH)2 area insertion. The a number of benefits of this insertion make it substitute for Phi29 Pol , particularly to be used in nanopore sequencing or different circumstances that require excessive salt concentrations.
swggeo
Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20600
Abclonal
5000U
EUR 49
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-01
Vazyme
1000 U
EUR 37.29
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ plus Buffer)
P101-03
Vazyme
10000 U
EUR 290
Taq DNA Polymerase (Mg2+ free Buffer)
P102-01
Vazyme
1000 U
EUR 145.2
Taq DNA Polymerase (Mg2+ free Buffer)
P102-02
Vazyme
5000 U
EUR 231.6
Taq DNA Polymerase (Mg2+ free Buffer)
P102-03
Vazyme
10000 U
EUR 314.4
Taq DNA Polymerase with dNTP Mix Buffer
11140015-1
Bio-WORLD
500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-2
Bio-WORLD
2500 Unit (s)
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Taq DNA Polymerase with dNTP Mix Buffer
11140015-3
Bio-WORLD
5000 Unit (s)
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HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)
RK20601
Abclonal
25 U
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Taq Polymerase (5U/µl)
MBT060A-1000U
EWC Diagnostics
1 unit
EUR 62.03
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-5000U
EWC Diagnostics
1 unit
EUR 241.01
Description: Taq Polymerase (5U/µl)
Taq Polymerase (5U/µl)
MBT060A-500U
EWC Diagnostics
1 unit
EUR 41.58
Description: Taq Polymerase (5U/µl)
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)
P101-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d1
Vazyme
1000 U
EUR 163.2
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d2
Vazyme
5000 U
EUR 320.4
Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)
P102-d3
Vazyme
10000 U
EUR 490.8
Taq DNA Polymerase, 5u/ul
B0089(D0089)
Bio Basic
5X200U
EUR 112.2
Taq Polymerase / Labeling Buffer
PCR-201L
Jena Bioscience GmbH
5x 200units
EUR 230.7
Description: Thermus aquaticus, recombinant, E. coli
Taq Polymerase / Labeling Buffer
PCR-201S
Jena Bioscience GmbH
200units
EUR 57.6
Description: Thermus aquaticus, recombinant, E. coli
HotStart Taq DNA Polymerase (5U/uL)
A4144-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Taq Plus DNA Polymerase, 5u/ul
D0090
Bio Basic
200U
EUR 94.8
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-1
Glycomatrix
500 Unit(s)
EUR 108.39
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-2
Glycomatrix
2500 Unit(s)
EUR 490.36
Taq DNA Polymerase with dNTP Mix, 10X Mg free Buffer and 25mM MgCl2
11140016-3
Glycomatrix
5000 Unit(s)
EUR 912.79
Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ001
Allele Biotech
1000U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ100
Allele Biotech
100U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ200
Allele Biotech
200U
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Taq DNA Polymerase, 5 units/µl
ABP-PP-ERTQ500
Allele Biotech
500U
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3G HotStart Taq DNA Polymerase (5u/ul)
3GHST81
Bio Basic
5x500U
EUR 896.24
Taq DNA Polymerase (High Purity), 5u/ul
HTD0078
Bio Basic
5X200U
EUR 129.6
AtMax Taq DNA Polymerase (2.5U/µl), 200U
PL4201
Vivantis
each
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AtMax Taq DNA Polymerase (2.5U/µl), 500U
PL4202
Vivantis
each
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Chromo Taq DNA Polymerase (1U/µl), 200U
PL1205
Vivantis
each
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Chromo Taq DNA Polymerase (1U/µl), 500U
PL1206
Vivantis
each
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DNA Polymerase Reaction Buffer
abx461036-1096tests
Abbexa
10 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-596tests
Abbexa
5 × 96 tests
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DNA Polymerase Reaction Buffer
abx461036-96tests
Abbexa
96 tests
EUR 562.5
Pfu DNA Polymerase (5U/µl), 100U
PL5201
Vivantis
each
Ask for price
Pfu DNA Polymerase (5U/µl), 500U
PL5202
Vivantis
each
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AtTaq DNA Polymerase (5U/µl), 200U
PL3201
Vivantis
each
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AtTaq DNA Polymerase (5U/µl), 500U
PL3202
Vivantis
each
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MaxTaq DNA Polymerase (5U/µl), 200U
PL2201
Vivantis
each
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MaxTaq DNA Polymerase (5U/µl), 500U
PL2202
Vivantis
each
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Long-Taq DNA Polymerase (5U/uL)
A4186-250U
Biomatik Corporation
250U
EUR 66
Description: Biotechnology
Taq DNA Polymerase
G009
ABM
1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.
Taq DNA polymerase
BT10101
Bioatlas
500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10102
Bioatlas
1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA polymerase
BT10103
Bioatlas
2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00103
Cusabio
500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00104
Bioatlas
1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
BA00105
Bioatlas
2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.
Taq DNA Polymerase
9001-2500
Biovision
each
EUR 614.4
Taq DNA Polymerase
9001-500
Biovision
each
EUR 229.2
Taq DNA Polymerase
L7051001
Biochain
1000 unit
EUR 167
Taq DNA Polymerase
L7051200
Biochain
200 unit
EUR 43
Taq DNA Polymerase
MB101-0500
Bio-Helix
500 U/100 μl
EUR 10
Taq DNA Polymerase
abx299725-100g
Abbexa
100 µg
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Taq DNA Polymerase
abx299725-20g
Abbexa
20 µg
EUR 337.5
Taq DNA Polymerase
abx299725-50g
Abbexa
50 µg
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Taq DNA Polymerase
abx461029-1096tests
Abbexa
10 × 96 tests
EUR 1787.5
Taq DNA Polymerase
abx461029-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase
abx461029-96tests
Abbexa
96 tests
EUR 362.5
Taq Polymerase(3U/µl)
MBT060B-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060B-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(3U/µl)
Taq Polymerase(3U/µl)
MBT060C-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(3U/µl)
Taq Polymerase(1U/µl)
MBT060D-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060D-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-1000U
EWC Diagnostics
1 unit
EUR 73.68
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-5000U
EWC Diagnostics
1 unit
EUR 286.42
Description: Taq Polymerase(1U/µl)
Taq Polymerase(1U/µl)
MBT060E-500U
EWC Diagnostics
1 unit
EUR 49.43
Description: Taq Polymerase(1U/µl)
h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-1
Bio-WORLD
250 Unit (s)
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h-Taq DNA Polymerase with dNTP Mix 10X Mg Free Buffer and 25mM MgCl
11140022-2
Bio-WORLD
1000 Unit (s)
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Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-01
Vazyme
125 U
EUR 145.2
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)
P301-02
Vazyme
500 U
EUR 210
Taq DNA Polymerase (3000U)
9K-001-0002
Bio Basic
3000U
EUR 582
Taq DNA Polymerase (6000U)
9K-001-0033
Bio Basic
6000U
EUR 1234.5
Taq DNA Polymerase (1000U)
9K-001-0035
Bio Basic
1000U
EUR 418.62
Taq DNA Polymerase (500U)
9K-001-0001
Bio Basic
500U
EUR 209.29
Taq HS DNA Polymerase
P132-d1
Vazyme
500 U
EUR 47.85
Taq HS DNA Polymerase
P132-d2
Vazyme
1,000 U
EUR 89.18
Taq HS DNA Polymerase
P132-d3
Vazyme
5,000 U
EUR 406
Taq DNA Polymerase (PCR)
G008
ABM
5000 U (1.0 ml)
EUR 246
Taq DNA Polymerase (PCR)
G126
ABM
10000 U (2 x 1.0 ml)
EUR 370.8
Taq DNA Polymerase Mix
abx461043-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq DNA Polymerase Mix
abx461043-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase Mix
abx461043-96tests
Abbexa
96 tests
EUR 337.5
Taq DNA polymerase (-dNTPs)
02-011
Sceti
200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (-dNTPs)
02-011-5
Sceti
5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001
Sceti
200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA polymerase (+dNTPs)
02-001-5
Sceti
5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.
Taq DNA Polymerase, 5units/㎕
T2200-050
GenDepot
500units
EUR 157.2
Taq DNA Polymerase, 5units/㎕
T2200-100
GenDepot
2x500 units
EUR 230.4
Taq DNA Polymerase, 5units/㎕
T2200-200
GenDepot
4x500 units
EUR 375.6
Maximo Taq DNA Polymerase
S101
GeneOn
500 units
EUR 55.2
Maximo Taq DNA Polymerase
S102
GeneOn
5x500 units
EUR 128.4
Maximo Taq DNA Polymerase
S103
GeneOn
20x500 units
EUR 393.6
Hotstart Taq DNA Polymerase
L7052001
Biochain
1000 units
EUR 595
Hotstart Taq DNA Polymerase
L7052250
Biochain
250 units
EUR 167
Taq DNA Polymerase -1000 units
3243
Intact Genomics
1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer
Champagne Taq DNA Polymerase
P122-d1
Vazyme
500 U (2.5 U/μl)
EUR 212.4
Champagne Taq DNA Polymerase
P122-d2
Vazyme
500 U (5 U/μl)
EUR 212.4
Champagne Taq DNA Polymerase
P122-d3
Vazyme
500 U (10 U/μl)
EUR 212.4
ACTaq? Taq DNA Polymerase, 1000U
E2100-1000U
ACTGene
each
EUR 260.4
Taq DNA Polymerase - 5,000 units
3245
Intact Genomics
1/EA
EUR 449
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer
Taq DNA Polymerase (Recombinant)
22060707-1
Glycomatrix
1,000 Unit(s)
EUR 137.27
Taq DNA Polymerase (Recombinant)
22060707-2
Glycomatrix
1500 Unit(s)
EUR 182.1
Taq DNA Polymerase (Recombinant)
22060707-3
Glycomatrix
5000 Unit(s)
EUR 465.77
Taq DNA Polymerase (Recombinant)
22060707-4
Glycomatrix
10 KU
EUR 840.96
Recombinant Taq DNA Polymerase
7-03634
CHI Scientific
1,000U
Ask for price
Recombinant Taq DNA Polymerase
7-03635
CHI Scientific
3,000U
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Recombinant Taq DNA Polymerase
7-03636
CHI Scientific
10,000U
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Taq DNA Polymerase (Recombinant)
abx073521-10000U
Abbexa
10000 U
EUR 710.4
Taq DNA Polymerase (Recombinant)
abx073521-1000U
Abbexa
1000 U
EUR 276
Taq DNA Polymerase (Recombinant)
abx073521-3000U
Abbexa
3000 U
EUR 393.6
Taq DNA Polymerase Recombinant
rAP-1062
Angio Proteomie
Inquiry
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ACTaq? Taq DNA Polymerase, 250U
E2100-250U
ACTGene
each
EUR 122.4
Taq Plus DNA Polymerase
P201-01
Vazyme
250 U
EUR 147.6
Taq Plus DNA Polymerase
P201-02
Vazyme
1000 U
EUR 213.6
Taq Plus DNA Polymerase
P201-03
Vazyme
3000 U
EUR 382.8
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)
P301-d2
Vazyme
500 U
EUR 213.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d1
Vazyme
125 U
EUR 147.6
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)
P302-d2
Vazyme
500 U
EUR 213.6
Taq Plus DNA Polymerase (Taq Plus DNA) Protein
abx073522-100tests
Abbexa
100 tests
EUR 225
Hot start Taq DNA Polymerase
EQ006
ELK Biotech
100μl/500U
EUR 200
Hot Start Taq DNA Polymerase
abx460010-1096tests
Abbexa
10 × 96 tests
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Hot Start Taq DNA Polymerase
abx460010-596tests
Abbexa
5 × 96 tests
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Hot Start Taq DNA Polymerase
abx460010-96tests
Abbexa
96 tests
EUR 262.5
Taq DNA Polymerase (TaqDNA) Protein
abx073521-100tests
Abbexa
100 tests
EUR 225
Apex Red Taq DNA Polymerase, 10000u
42-402R
Genesee Scientific
20 x 500u/Unit
EUR 1229.49
Description: 10X NH4 Buffer, Mg Free
Apex Red Taq DNA Polymerase, 500u
42-409R
Genesee Scientific
1 x 500u/Unit
EUR 62.1
Description: 10X NH4 Buffer, Mg Free
Taq HS Red DNA Polymerase
abx461041-1096tests
Abbexa
10 × 96 tests
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Taq HS Red DNA Polymerase
abx461041-596tests
Abbexa
5 × 96 tests
EUR 2187.5
Taq HS Red DNA Polymerase
abx461041-96tests
Abbexa
96 tests
EUR 1062.5
Taq Plus DNA Polymerase (PCR)
G012
ABM
250 U (50 ul)
EUR 87.6
Taq Plus DNA Polymerase (PCR)
G040
ABM
1000 U (200 ul)
EUR 127.2
Taq DNA Polymerase Polyclonal Antibody
7018-100
Biovision
each
EUR 424.8
Taq DNA Polymerase Polyclonal Antibody
7018-30T
Biovision
each
EUR 175.2
AffiTaq® DNA Polymerase - With Buffer - With MgCl2 - Without dNTP
AFG-BOR-101
AffiGEN
10X (1000U/200µL)
EUR 380
Taq DNA Polymerase with Red Dye
abx461047-1096tests
Abbexa
10 × 96 tests
EUR 1787.5
Taq DNA Polymerase with Red Dye
abx461047-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase with Red Dye
abx461047-96tests
Abbexa
96 tests
EUR 362.5
ACTaq? Taq Blue DNA Polymerase, 1000U
E2100B-1000U
ACTGene
each
EUR 272.4
Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled
AD-146-U
Alpha Diagnostics
Custom
Ask for price
ACTaq? Taq Blue DNA Polymerase, 250U
E2100B-250U
ACTGene
each
EUR 126
Taq HS DNA Polymerase (Glycerol-free)
QL101-01
Vazyme
1,000 U
EUR 89
Taq HS DNA Polymerase (Glycerol-free)
QL101-02
Vazyme
5,000 U
EUR 406
Taq HS DNA Polymerase (Glycerol-free)
QL101-03
Vazyme
50,000 U
EUR 3625
Recombinant Taq Plus DNA Polymerase
7-03631
CHI Scientific
250U
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Recombinant Taq Plus DNA Polymerase
7-03632
CHI Scientific
750U
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Recombinant Taq Plus DNA Polymerase
7-03633
CHI Scientific
2,500U
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Taq Plus DNA Polymerase (Recombinant)
abx073522-2500U
Abbexa
2.500 U
EUR 710.4
Taq Plus DNA Polymerase (Recombinant)
abx073522-250U
Abbexa
250 U
EUR 276
Taq Plus DNA Polymerase (Recombinant)
abx073522-750U
Abbexa
750 U
EUR 393.6
Taq Plus DNA Polymerase Recombinant
rAP-1063
Angio Proteomie
Inquiry
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Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B01
GenDepot
10,000 units
EUR 1388.4
Taq DNA Polymerase, 5units/㎕ , Bulk Packing
T2200-B05
GenDepot
5x10,000 units
EUR 6540
Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated
AD-146-B
Alpha Diagnostics
Custom
Ask for price
Taq DNA Polymerase M101 (5,000 U/mL)
RK26000
Abclonal
250U
EUR 60
amfiSure Hot-Start Taq DNA Polymerase
P0322-010
GenDepot
100 units
EUR 176.4
amfiSure Hot-Start Taq DNA Polymerase
P0322-025
GenDepot
250 units
EUR 273.6
T4 DNA Polymerase, 5u/ul
BEP0061
Bio Basic
100U
EUR 153.97
DNA Amplification Kit with Taq DNA Polymerase, 100app
PL1202-K
Vivantis
each
Ask for price
Tsg DNA Polymerase, 5u/ul
D0081
Bio Basic
5X200U
EUR 112.2
Pfu DNA Polymerase, 5u/ul
B0093(D0090P)
Bio Basic
500U
EUR 164.4
Taq DNA Polymerase with Red Dye Mix
abx461048-1096tests
Abbexa
10 × 96 tests
Ask for price
Taq DNA Polymerase with Red Dye Mix
abx461048-596tests
Abbexa
5 × 96 tests
EUR 1062.5
Taq DNA Polymerase with Red Dye Mix
abx461048-96tests
Abbexa
96 tests
EUR 325
Taq DNA Polymerase with dNTP Mix (6000U)
9K-001-0018
Bio Basic
6000U
EUR 1536.74
Fe-S coordination defects within the replicative DNA polymerase delta trigger deleterious DNA replication in vivo and subsequent DNA harm within the yeast Saccharomyces cerevisiae
B-type eukaryotic polymerases comprise a [4Fe-4S] cluster of their C-terminus area, whose function shouldn’t be totally understood but. Amongst them, DNA polymerase delta (Polδ) performs a vital function in chromosomal DNA replication, largely throughout lagging strand synthesis. Earlier in vitro work recommended that the Fe-S cluster in Polδ is required for environment friendly binding of the Pol31 subunit, guaranteeing stability of the Polδ complicated. Right here we analyzed the in vivo penalties ensuing from an impaired coordination of the Fe-S cluster in Polδ.
We present {that a} single substitution of the final cysteine coordinating the cluster by a serine is answerable for the era of huge DNA harm throughout S section , resulting in checkpoint activation, requirement of homologous recombination for restore, and finally to cell loss of life when the restore capacities of the cells are overwhelmed. These information point out that impaired Fe-S cluster coordination in Polδ is answerable for aberrant replication. Extra usually, Fe-S in Polδ could also be compromised by numerous stress together with anti-cancer medication. Doable in vivo Polδ Fe-S cluster oxidation and collapse might thus happen, and we speculate this might contribute to induced genomic instability and cell loss of life, similar to that noticed in pol3-13 cells.
Ultrafast and Actual-Time Nanoplasmonic On-Chip Polymerase Chain Response for Fast and Quantitative Molecular Diagnostics
Introduction and quick unfold of pandemic ailments draw worldwide consideration to fast, immediate, and correct molecular diagnostics with technical improvement of ultrafast polymerase chain response (PCR). Microfluidic on-chip PCR platforms present extremely environment friendly and small-volume bioassay for point-of-care diagnostic functions. Right here we report ultrafast, real-time , and on-chip nanoplasmonic PCR for fast and quantitative molecular diagnostics at point-of-care degree.
The plasmofluidic PCR chip contains glass nanopillar arrays with Au nanoislands and gas-permeable microfluidic channels, which comprise response microchamber arrays, a precharged vacuum cell, and a vapor barrier. The on-chip configuration permits each spontaneous pattern loading and microbubble-free PCR response throughout which the plasmonic nanopillar arrays end in ultrafast photothermal biking. After fast pattern loading lower than three min, two-step PCR outcomes for 40 cycles present fast amplification in 264 s for lambda-DNA, and 306 s for plasmids expressing SARS-CoV-2 envelope protein. As well as, the in situ cyclic real-time quantification of amplicons clearly demonstrates the amplification efficiencies of greater than 91%. This PCR platform can present fast point-of-care molecular diagnostics in serving to sluggish the fast-spreading pandemic.
A microfluidic-integrated lateral stream recombinase polymerase amplification (MI-IF-RPA) assay for fast COVID-19 detection
The COVID-19 pandemic, attributable to SARS-CoV-2 , at present poses an pressing world medical disaster for which there stays an absence of inexpensive point-of-care testing (POCT). Specifically, resource-limited areas want easy and simply disseminated testing options to handle the outbreak. On this work, a microfluidic-integrated lateral stream recombinase polymerase amplification (MI-IF-RPA) assay was developed for fast and delicate detection of SARS-CoV-2 , which integrates the reverse transcription recombinase polymerase amplification (RT-RPA) and a common lateral stream (LF) dipstick detection system right into a single microfluidic chip.
The only-chamber RT-RPA response parts are blended with operating buffer, after which delivered to the LF detection strips for biotin- and FAM-labelled amplified analyte sequences, which might present simply interpreted constructive or adverse outcomes. Testing requires solely a easy nucleic acid extraction and loading, then incubation to acquire outcomes, roughly 30 minutes in whole. SARS-CoV-2 armored RNA particles have been used to validate the MI-IF-RPA system, which confirmed a restrict of detection of 1 copy per μL, or 30 copies per pattern.
Chip efficiency was additional evaluated utilizing clinically identified circumstances of COVID-19 and revealed a sensitivity of 97% and specificity of 100%, extremely similar to present reverse transcription-polymerase chain response (RT-PCR)-based diagnostic assays. This MI-IF-RPA assay is moveable and contains inexpensive supplies, enabling mass manufacturing and decreased threat of contamination. With out the necessity for specialised instrumentation and coaching, MI-IF-RPA assay can be utilized as a complement to RT-PCR for low-cost COVID-19 screening in resource-limited areas.
A DNA polymerization-independent function for mitochondrial DNA polymerase IC in African trypanosomes
The mitochondrial DNA of Trypanosoma brucei and associated parasites is a catenated community containing 1000’s of minicircles and tens of maxicircles referred to as kinetoplast DNA (kDNA). Replication of the one nucleoid requires at the very least three DNA polymerases (POLIB, POLIC, and POLID) every having discrete localization close to the kDNA throughout S section. POLIB and POLID have roles in minicircle replication whereas the precise function of POLIC in kDNA upkeep is much less clear. Right here, we use an RNAi-complementation system to dissect the features of the distinct POLIC domains: the conserved household A DNA polymerase area (POLA) and the uncharacterized N-terminal area (UCR) .
Whereas RNAi complementation with wild-type POLIC restored kDNA content material and cell cycle localization, energetic website level mutations within the POLA area impaired minicircle replication equally to POLIB and POLID depletions. Complementation with POLA area alone abolished POLIC foci formation and partially rescued the RNAi phenotype. Moreover, we offer proof of a essential function for the UCR in cell cycle localization that facilitates correct distribution of progeny networks. That is the primary report of a DNA polymerase that impacts mitochondrial nucleoid distribution.
Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in S. pombe
Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that guarantee cell survival by facilitating DNA replication within the presence of DNA harm. Along with their function in bypassing lesions, TLSPs have been implicated in meiotic double strand break restore in a number of methods. Right here we study the joint contribution of 4 TLS polymerases to meiotic development within the fission yeast S. pombe.
We noticed the dramatic lack of spore viability in fission yeast missing all 4 TLSPs which is accompanied by disruptions in chromosome segregation throughout meiosis I and II. Rec8 cohesin dynamics are altered in the absence of the TLSPs . These information counsel that the TLSPs contribute to a number of points of meiotic chromosome dynamics.
DNA Polymerase Lambda (POLL) Antibody
abx034579-400ul
Abbexa
400 ul
EUR 627.6
DNA Polymerase Lambda (POLL) Antibody
abx034579-80l
Abbexa
80 µl
EUR 343.2
DNA Polymerase Lambda (POLL) Antibody
20-abx217850
Abbexa
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DNA Polymerase Lambda (POLL) Antibody
20-abx005922
Abbexa
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100 ul
200 ul
20 ul
50 ul
DNA Polymerase Lambda (POLL) Antibody
20-abx013831
Abbexa
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100 ug
10 ug
200 ug
300 µg
DNA Polymerase Lambda (POLL) Antibody
abx013831-100l
Abbexa
100 µl
EUR 43.75
DNA Polymerase Lambda (POLL) Antibody
abx005922-100l
Abbexa
100 µl
EUR 175
DNA Polymerase Lambda (POLL) Antibody
abx301115-100g
Abbexa
100 µg
EUR 362.5
DNA Polymerase Lambda (POLL) Antibody
abx301115-20g
Abbexa
20 µg
EUR 162.5
DNA Polymerase Lambda (POLL) Antibody
abx301115-50g
Abbexa
50 µg
EUR 250
DNA Polymerase Lambda (POLL) Antibody
abx034579-100g
Abbexa
100 µg
EUR 250
DNA Polymerase Lambda (POLL) Antibody
abx239963-100l
Abbexa
100 µl
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DNA Polymerase Lambda (POLL) Antibody
abx239963-50l
Abbexa
50 µl
EUR 350
DNA Polymerase Lambda (POLL) Antibody
abx328341-100g
Abbexa
100 µg
EUR 250
DNA Polymerase Lambda (POLL) Antibody
abx328341-50g
Abbexa
50 µg
EUR 187.5
DNA Polymerase Lambda (POLL) Antibody
abx430146-200l
Abbexa
200 µl
EUR 387.5
DNA Polymerase Lambda (POLL) Antibody (HRP)
20-abx317655
Abbexa
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100 ug
1 mg
200 ug
20 ug
50 ug
DNA Polymerase Lambda (POLL) Antibody (HRP)
abx317655-100g
Abbexa
100 µg
EUR 362.5
DNA Polymerase Lambda (POLL) Antibody (HRP)
abx317655-20g
Abbexa
20 µg
EUR 162.5
DNA Polymerase Lambda (POLL) Antibody (HRP)
abx317655-50g
Abbexa
50 µg
EUR 250
DNA Polymerase Lambda (POLL) Antibody (FITC)
20-abx317656
Abbexa
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100 ug
1 mg
200 ug
20 ug
50 ug
DNA Polymerase Lambda (POLL) Antibody (FITC)
abx317656-100g
Abbexa
100 µg
EUR 362.5
DNA Polymerase Lambda (POLL) Antibody (FITC)
abx317656-20g
Abbexa
20 µg
EUR 162.5
DNA Polymerase Lambda (POLL) Antibody (FITC)
abx317656-50g
Abbexa
50 µg
EUR 250
DNA Polymerase Lambda (POLL) Antibody (Biotin)
20-abx317657
Abbexa
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100 ug
1 mg
200 ug
20 ug
50 ug
DNA Polymerase Lambda (POLL) Antibody (Biotin)
abx317657-100g
Abbexa
100 µg
EUR 362.5
DNA Polymerase Lambda (POLL) Antibody (Biotin)
abx317657-20g
Abbexa
20 µg
EUR 162.5
DNA Polymerase Lambda (POLL) Antibody (Biotin)
abx317657-50g
Abbexa
50 µg
EUR 250
Rabbit anti-DNA Polymerase lambda Antibody
DL99270A-100ul
DL Develop
100 ul
EUR 299
Description: DNA polymerase lambda; Pol Lambda; DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Rabbit anti-DNA Polymerase lambda Antibody
DL99270A-50ul
DL Develop
50 ul
EUR 210
Description: DNA polymerase lambda; Pol Lambda; DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Anti-DNA Polymerase lambda/POLL Antibody
A00959-2
BosterBio
100ug/vial
EUR 352.8
Human DNA Polymerase Lambda (POLL) Protein
abx073724-100tests
Abbexa
100 tests
EUR 225
Human DNA Polymerase Lambda (POLL) Protein
abx073724-1920tests
Abbexa
1920 tests
EUR 6575
Human DNA Polymerase Lambda (POLL) Protein
abx073724-400tests
Abbexa
400 tests
EUR 325
Rat DNA Polymerase Lambda (POLL) ELISA Kit
abx510952-96tests
Abbexa
96 tests
EUR 687.5
Mouse DNA polymerase lambda, Poll ELISA KIT
ELI-47091m
Lifescience Market
96 Tests
EUR 1038
Human DNA polymerase lambda, POLL ELISA KIT
ELI-08853h
Lifescience Market
96 Tests
EUR 988.8
Human DNA polymerase lambda (POLL) ELISA Kit
AE26697HU-48Tests
Abebio
48 Tests
EUR 325
Description: Human (Homo sapiens)
Human DNA polymerase lambda (POLL) ELISA Kit
AE26697HU-96Tests
Abebio
96 Tests
EUR 610
Description: Human (Homo sapiens)
Human DNA Polymerase Lambda (POLL) ELISA Kit
abx510950-96tests
Abbexa
96 tests
EUR 687.5
Mouse DNA Polymerase Lambda (POLL) ELISA Kit
abx510951-96tests
Abbexa
96 tests
EUR 687.5
Polymerase (DNA directed), lambda (Recombinant)
20-abx073724
Abbexa
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DNA Polymerase lambda (POLL) (C-term) rabbit polyclonal antibody, Purified
AP53380PU-N
Origene Technologies GmbH
400 µl
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DNA Polymerase lambda (POLL) (563-575) goat polyclonal antibody, Aff - Purified
AP08560PU-N
Origene Technologies GmbH
50 µg
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Polymerase (DNA directed), Lambda Human Recombinant
rAP-1590
Angio Proteomie
Inquiry
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Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
DL87223A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
DL87223A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
E38A3245
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
YLF0515-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
YLF0515-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 488
YLF0515-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Polymerase (DNA Directed) Lambda (POLL) Goat anti-Human Polyclonal (C-Terminus) Antibody
GWB-D31402
GenWay Biotech
0.05 mg
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POLL Polymerase (DNA directed), Lambda Human Recombinant Protein
PROTQ9UGP5
BosterBio
Regular: 10ug
EUR 380.4
Description: POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.;POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Poll (untagged ORF) - Rat polymerase (DNA directed), lambda (Poll), (10 ug)
RN211260
Origene Technologies GmbH
10 µg
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Poll (Myc-DDK-tagged) - Mouse polymerase (DNA directed), lambda (Poll)
MR209017
Origene Technologies GmbH
10 µg
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Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
DL87137A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
DL87137A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
DL87180A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
DL87180A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
DL87266A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
DL87266A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
DL87309A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
DL87309A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
DL87352A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
DL87352A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
DL87395A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
DL87395A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
DL87438A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
DL87438A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
DL87481A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
DL87481A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
DL87524A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
DL87524A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
DL87567A-100ul
DL Develop
100 ul
EUR 190
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
DL87567A-500ul
DL Develop
500 ul
EUR 280
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
DL87610A-100ul
DL Develop
100 ul
EUR 232
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
DL87610A-500ul
DL Develop
500 ul
EUR 320
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
DL87653A-100ul
DL Develop
100 ul
EUR 232
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
DL87653A-500ul
DL Develop
500 ul
EUR 320
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
E38A3945
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
E38A4045
EnoGene
100ug/100ul
EUR 125
Description: Available in various conjugation types.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
E38A4145
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
E38A4245
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
E38A3045
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
E38A3145
EnoGene
100ug/100ul
EUR 125
Description: Available in various conjugation types.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
E38A3345
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
E38A3445
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
E38A3545
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
E38A3645
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
E38A3845
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
E38A3745
EnoGene
100μL
EUR 125
Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
YLF0487-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
YLF0487-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 350
YLF0487-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
YLF0501-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
YLF0501-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 405
YLF0501-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
YLF0529-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
YLF0529-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 532
YLF0529-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
YLF0543-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
YLF0543-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 555
YLF0543-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
YLF0557-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
YLF0557-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 568
YLF0557-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
YLF0571-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
YLF0571-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 594
YLF0571-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
YLF0585-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
YLF0585-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 633
YLF0585-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
YLF0599-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
YLF0599-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 647
YLF0599-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
YLF0613-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
YLF0613-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 660
YLF0613-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
YLF0627-100ul
Shanghai YL Biotech
100 ul
EUR 130
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
YLF0627-1ml
Shanghai YL Biotech
1 ml
EUR 300
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 680
YLF0627-500ul
Shanghai YL Biotech
500 ul
EUR 210
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
YLF0641-100ul
Shanghai YL Biotech
100 ul
EUR 160
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
YLF0641-1ml
Shanghai YL Biotech
1 ml
EUR 370
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 750
YLF0641-500ul
Shanghai YL Biotech
500 ul
EUR 240
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
YLF0655-100ul
Shanghai YL Biotech
100 ul
EUR 160
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
YLF0655-1ml
Shanghai YL Biotech
1 ml
EUR 370
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Mouse Anti-Human Ig lambda chain - Alexa Fluor 790
YLF0655-500ul
Shanghai YL Biotech
500 ul
EUR 240
Description: The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
Poll (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), (10 ug)
RR211260
Origene Technologies GmbH
10 µg
Ask for price
POLL (untagged)-Human polymerase (DNA directed) lambda (POLL) transcript variant 3
SC328795
Origene Technologies GmbH
10 µg
Ask for price
POLL (untagged)-Human polymerase (DNA directed) lambda (POLL) transcript variant 1
SC328936
Origene Technologies GmbH
10 µg
Ask for price
POLL (untagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2
SC115324
Origene Technologies GmbH
10 µg
Ask for price
Lenti ORF clone of Poll (mGFP-tagged) - Mouse polymerase (DNA directed), lambda (Poll)
MR209017L4
Origene Technologies GmbH
10 µg
Ask for price
3`UTR clone of polymerase (DNA directed) lambda (POLL) for miRNA target validation
SC207720
Origene Technologies GmbH
10 µg
Ask for price
POLL (GFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 2
RG209816
Origene Technologies GmbH
10 µg
Ask for price
POLL (GFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 1
RG230298
Origene Technologies GmbH
10 µg
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POLL (GFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 3
RG230157
Origene Technologies GmbH
10 µg
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Poll (untagged) - Mouse polymerase (DNA directed), lambda (cDNA clone MGC:7039 IMAGE:3156259), (10ug)
MC206449
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Poll (Myc-DDK-tagged) - Mouse polymerase (DNA directed), lambda (Poll)
MR209017L3
Origene Technologies GmbH
10 µg
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POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 1
RC230298
Origene Technologies GmbH
10 µg
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POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 3
RC230157
Origene Technologies GmbH
10 µg
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POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2
RC209816
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Poll (mGFP-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), (10 ug)
RR211260L4
Origene Technologies GmbH
10 µg
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Anti-Hu Ig Lambda Light Chain Alexa Fluor® 700
A7-673-T025
ExBio
25 tests
EUR 121
Anti-Hu Ig Lambda Light Chain Alexa Fluor® 700
A7-673-T100
ExBio
100 tests
EUR 242
DNA Polymerase lamda Antibody
34098
SAB
100ul
EUR 319
Lenti ORF clone of Poll (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), (10 ug)
RR211260L3
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, mGFP tagged
RC230157L4
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, mGFP tagged
RC209816L4
Origene Technologies GmbH
10 µg
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Lenti ORF particles, Poll (GFP-tagged) - Mouse polymerase (DNA directed), lambda (Poll), 200ul, >10^7 TU/mL
MR209017L4V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, Poll (GFP-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), 200ul, >10^7 TU/mL
RR211260L4V
Origene Technologies GmbH
200 µl
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Lenti ORF particles, Poll (Myc-DDK-tagged) - Mouse polymerase (DNA directed), lambda (Poll), 200ul, >10^7 TU/mL
MR209017L3V
Origene Technologies GmbH
200 µl
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Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 3, Myc-DDK-tagged
RC230157L3
Origene Technologies GmbH
10 µg
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Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, Myc-DDK-tagged
RC209816L3
Origene Technologies GmbH
10 µg
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Lenti-ORF clone of POLL (mGFP-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 1
RC230298L4
Origene Technologies GmbH
10 µg
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Lenti ORF particles, Poll (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), lambda (Poll), 200ul, >10^7 TU/mL
RR211260L3V
Origene Technologies GmbH
200 µl
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DNA Polymerase lamda Conjugated Antibody
C34098
SAB
100ul
EUR 476.4
DNA Polymerase Antibody
E11-11836C
EnoGene
100μg
EUR 225
Description: Available in various conjugation types.
DNA Polymerase Antibody
8C10144
AAT Bioquest
50ug
EUR 368
Description: DNA Polymerase ζ Antibody
Lenti-ORF clone of POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 1
RC230298L3
Origene Technologies GmbH
10 µg
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EGFR antibody (Alexa Fluor 488)
61R-E109BAF
Fitzgerald
125 ug
EUR 714
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)
DNA Polymerase β Antibody
33357
SAB
100ul
EUR 319
DNA Polymerase β Antibody
33357-100ul
SAB
100ul
EUR 302.4
DNA Polymerase β Antibody
33357-50ul
SAB
50ul
EUR 224.4
DNA Polymerase ζ Antibody
33602
SAB
100ul
EUR 319
DNA Polymerase ζ Antibody
33602-100ul
SAB
100ul
EUR 302.4
DNA Polymerase ζ Antibody
33602-50ul
SAB
50ul
EUR 224.4
DNA Polymerase θ Antibody
33611
SAB
100ul
EUR 319
DNA Polymerase θ Antibody
33611-100ul
SAB
100ul
EUR 302.4
DNA Polymerase θ Antibody
33611-50ul
SAB
50ul
EUR 224.4
DNA Polymerase α Antibody
33665
SAB
100ul
EUR 319
DNA Polymerase α Antibody
33665-100ul
SAB
100ul
EUR 302.4
DNA Polymerase α Antibody
33665-50ul
SAB
50ul
EUR 224.4
DNA Polymerase λ Antibody
34098-100ul
SAB
100ul
EUR 302.4
DNA Polymerase λ Antibody
34098-50ul
SAB
50ul
EUR 224.4
DNA Polymerase β Antibody
E033357
EnoGene
100μg/100μl
EUR 255
Description: Available in various conjugation types.
DNA Polymerase ζ Antibody
E033602
EnoGene
100μg/100μl
EUR 255
Description: Available in various conjugation types.
DNA Polymerase θ Antibody
E033611
EnoGene
100μg/100μl
EUR 255
Description: Available in various conjugation types.
DNA Polymerase α Antibody
E033665
EnoGene
100μg/100μl
EUR 255
Description: Available in various conjugation types.
DNA Polymerase λ Antibody
E034098
EnoGene
100μg/100μl
EUR 255
Description: Available in various conjugation types.
DNA Polymerase α Antibody
E19-3082
EnoGene
100μg/100μl
EUR 225
Description: Available in various conjugation types.
DNA Polymerase α Antibody
E18-5235-1
EnoGene
50μg/50μl
EUR 145
Description: Available in various conjugation types.
DNA Polymerase α Antibody
E18-5235-2
EnoGene
100μg/100μl
EUR 225
Description: Available in various conjugation types.
DNA Polymerase ζ Antibody
E11-10144C
EnoGene
100μg/100μl
EUR 225
Description: Available in various conjugation types.
DNA Polymerase θ Antibody
E11-10173C
EnoGene
100μg
EUR 225
Description: Available in various conjugation types.
DNA Polymerase α Antibody
E11-10322C
EnoGene
100μg
EUR 225
Description: Available in various conjugation types.
DNA Polymerase β Antibody
E11-0173C
EnoGene
100μg/100μl
EUR 225
Description: Available in various conjugation types.
DNA Polymerase λ Antibody
8C11836
AAT Bioquest
50ug
EUR 368
Description: DNA Polymerase λ Antibody
DNA Polymerase β Antibody
8C0173
AAT Bioquest
50ug
EUR 368
Description: DNA Polymerase β Antibody
DNA Polymerase θ Antibody
8C10173
AAT Bioquest
50ug
EUR 368
Description: DNA Polymerase θ Antibody
DNA Polymerase α Antibody
8C10322
AAT Bioquest
50ug
EUR 368
Description: DNA Polymerase α Antibody
DNA Polymerase beta Antibody
35404
SAB
100ul
EUR 479
DNA Polymerase beta Antibody
35404-100ul
SAB
100ul
EUR 468
DNA Polymerase beta Antibody
E18-0148-1
EnoGene
50μg/50μl
EUR 145
Description: Available in various conjugation types.