Chimeric Phi29 DNA polymerase with helix-hairpin-helix

Chimeric Phi29 DNA polymerase with helix-hairpin-helix motifs exhibits enhanced salt tolerance and replication efficiency

Phi29 DNA polymerase (Phi29 Pol) has been efficiently utilized in DNA nanoball-based sequencing, real-time DNA sequencing from single polymerase molecules and nanopore sequencing using the sequencing by synthesis (SBS) technique. Amongst these, polymerase-assisted nanopore sequencing expertise analyses nucleotide sequences as a operate of modifications in electrical present. This ionic, current-based sequencing expertise requires polymerases to carry out replication at excessive salt concentrations, for instance 0.three M KCl. Nonetheless, the salt tolerance of wild-type Phi29 Pol is comparatively low.

Right here, we fused helix-hairpin-helix (HhH)2 domains E-L (eight repeats in whole) of topoisomerase V (Topo V) from the hyperthermophile Methanopyrus kandleri to the Phi29 Pol COOH terminus, designated Phi29EL DNA polymerase (Phi29EL Pol). Area fusion elevated the general enzyme replication effectivity by fourfold. Phi29EL Pol catalysed rolling circle replication in a broader vary of salt concentrations than did Phi29 Pol, extending the KCl focus vary for exercise as much as 0.three M. As well as, the mutation of Glu375 to Ser or Gln elevated Phi29EL Pol exercise within the presence of KCl. On this work, we produced a salt-tolerant Phi29 Pol by-product by the use of (HhH)2 area insertion. The a number of benefits of this insertion make it substitute for Phi29 Pol, particularly to be used in nanopore sequencing or different circumstances that require excessive salt concentrations.

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Taq DNA Polymerase (Mg2+ plus Buffer)

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Taq DNA Polymerase (Mg2+ plus Buffer)

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Taq DNA Polymerase (Mg2+ free Buffer)

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EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Hot Start Taq DNA Polymerase - 1000 units

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Taq DNA Polymerase 2x Premix - 500 Reactions

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amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

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Taq DNA Polymerase with dNTP Mix (6000U)

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Accuris Taq Polymerase

PR1000-1000 1 PC
EUR 172.66
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EUR 618.47
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Accuris Taq Polymerase

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10x Taq Buffer (MgCl2)

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Taq DNA Polymerase, Ultra Pure, 5 units/ul

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Taq DNA Polymerase, Ultra Pure, 5 units/ul

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HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

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HiQTM Taq Red DNA Polymerase, 1unit/㎕ , Bulk Packing

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Tsg PlusDNA Polymerase, 5u/ul

D0088 200U
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Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-01 125 U
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Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 175

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
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DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
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SDS-PAGE Sample Loading Buffer (6x)

abx090649-5ml 5 ml
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1.2ML Triple Dye Loading Buffer (6X)

NAT1192 1.2ML
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Taq DNA Polymerase - 5,000 units with separate dNTP Mix

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amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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amfiSure Taq DNA Polymerase, 1unit/ul, Including dNTP&MgCl2

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S113 2x100 rcs
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S400 200 units
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S410 1000 units
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Fe-S coordination defects within the replicative DNA polymerase delta trigger deleterious DNA replication in vivo and subsequent DNA harm within the yeast Saccharomyces cerevisiae

B-type eukaryotic polymerases comprise a [4Fe-4S] cluster of their C-terminus area, whose function shouldn’t be totally understood but. Amongst them, DNA polymerase delta (Polδ) performs a vital function in chromosomal DNA replication, largely throughout lagging strand synthesis. Earlier in vitro work recommended that the Fe-S cluster in Polδ is required for environment friendly binding of the Pol31 subunit, guaranteeing stability of the Polδ complicated. Right here we analyzed the in vivo penalties ensuing from an impaired coordination of the Fe-S cluster in Polδ.

We present {that a} single substitution of the final cysteine coordinating the cluster by a serine is answerable for the era of huge DNA harm throughout S section, resulting in checkpoint activation, requirement of homologous recombination for restore, and finally to cell loss of life when the restore capacities of the cells are overwhelmed. These information point out that impaired Fe-S cluster coordination in Polδ is answerable for aberrant replication. Extra usually, Fe-S in Polδ could also be compromised by numerous stress together with anti-cancer medication. Doable in vivo Polδ Fe-S cluster oxidation and collapse might thus happen, and we speculate this might contribute to induced genomic instability and cell loss of life, similar to that noticed in pol3-13 cells.

Ultrafast and Actual-Time Nanoplasmonic On-Chip Polymerase Chain Response for Fast and Quantitative Molecular Diagnostics

Introduction and quick unfold of pandemic ailments draw worldwide consideration to fast, immediate, and correct molecular diagnostics with technical improvement of ultrafast polymerase chain response (PCR). Microfluidic on-chip PCR platforms present extremely environment friendly and small-volume bioassay for point-of-care diagnostic functions. Right here we report ultrafast, real-time, and on-chip nanoplasmonic PCR for fast and quantitative molecular diagnostics at point-of-care degree.

The plasmofluidic PCR chip contains glass nanopillar arrays with Au nanoislands and gas-permeable microfluidic channels, which comprise response microchamber arrays, a precharged vacuum cell, and a vapor barrier. The on-chip configuration permits each spontaneous pattern loading and microbubble-free PCR response throughout which the plasmonic nanopillar arrays end in ultrafast photothermal biking. After fast pattern loading lower than three min, two-step PCR outcomes for 40 cycles present fast amplification in 264 s for lambda-DNA, and 306 s for plasmids expressing SARS-CoV-2 envelope protein. As well as, the in situ cyclic real-time quantification of amplicons clearly demonstrates the amplification efficiencies of greater than 91%. This PCR platform can present fast point-of-care molecular diagnostics in serving to sluggish the fast-spreading pandemic.

A microfluidic-integrated lateral stream recombinase polymerase amplification (MI-IF-RPA) assay for fast COVID-19 detection

The COVID-19 pandemic, attributable to SARS-CoV-2, at present poses an pressing world medical disaster for which there stays an absence of inexpensive point-of-care testing (POCT). Specifically, resource-limited areas want easy and simply disseminated testing options to handle the outbreak. On this work, a microfluidic-integrated lateral stream recombinase polymerase amplification (MI-IF-RPA) assay was developed for fast and delicate detection of SARS-CoV-2, which integrates the reverse transcription recombinase polymerase amplification (RT-RPA) and a common lateral stream (LF) dipstick detection system right into a single microfluidic chip.

The only-chamber RT-RPA response parts are blended with operating buffer, after which delivered to the LF detection strips for biotin- and FAM-labelled amplified analyte sequences, which might present simply interpreted constructive or adverse outcomes. Testing requires solely a easy nucleic acid extraction and loading, then incubation to acquire outcomes, roughly 30 minutes in whole. SARS-CoV-2 armored RNA particles have been used to validate the MI-IF-RPA system, which confirmed a restrict of detection of 1 copy per μL, or 30 copies per pattern.

Chip efficiency was additional evaluated utilizing clinically identified circumstances of COVID-19 and revealed a sensitivity of 97% and specificity of 100%, extremely similar to present reverse transcription-polymerase chain response (RT-PCR)-based diagnostic assays. This MI-IF-RPA assay is moveable and contains inexpensive supplies, enabling mass manufacturing and decreased threat of contamination. With out the necessity for specialised instrumentation and coaching, MI-IF-RPA assay can be utilized as a complement to RT-PCR for low-cost COVID-19 screening in resource-limited areas.

A DNA polymerization-independent function for mitochondrial DNA polymerase IC in African trypanosomes

The mitochondrial DNA of Trypanosoma brucei and associated parasites is a catenated community containing 1000’s of minicircles and tens of maxicircles referred to as kinetoplast DNA (kDNA). Replication of the one nucleoid requires at the very least three DNA polymerases (POLIB, POLIC, and POLID) every having discrete localization close to the kDNA throughout S section. POLIB and POLID have roles in minicircle replication whereas the precise function of POLIC in kDNA upkeep is much less clear. Right here, we use an RNAi-complementation system to dissect the features of the distinct POLIC domains: the conserved household A DNA polymerase area (POLA) and the uncharacterized N-terminal area (UCR).

Whereas RNAi complementation with wild-type POLIC restored kDNA content material and cell cycle localization, energetic website level mutations within the POLA area impaired minicircle replication equally to POLIB and POLID depletions. Complementation with POLA area alone abolished POLIC foci formation and partially rescued the RNAi phenotype. Moreover, we offer proof of a essential function for the UCR in cell cycle localization that facilitates correct distribution of progeny networks. That is the primary report of a DNA polymerase that impacts mitochondrial nucleoid distribution.

Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in S. pombe

Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that guarantee cell survival by facilitating DNA replication within the presence of DNA harm. Along with their function in bypassing lesions, TLSPs have been implicated in meiotic double strand break restore in a number of methods. Right here we study the joint contribution of 4 TLS polymerases to meiotic development within the fission yeast S. pombe.

We noticed the dramatic lack of spore viability in fission yeast missing all 4 TLSPs which is accompanied by disruptions in chromosome segregation throughout meiosis I and II. Rec8 cohesin dynamics are altered in the absence of the TLSPs. These information counsel that the TLSPs contribute to a number of points of meiotic chromosome dynamics.

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-RPSA Alexa Fluor® 488

A4-829-C100 0.1 mg
EUR 310

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 100 tests
EUR 269

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase Lambda (POLL) Antibody

abx034579-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

abx034579-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

20-abx005922
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

20-abx013831
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

20-abx328341
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

20-abx217850
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

abx332616-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody

abx430146-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

DNA Polymerase Lambda (POLL) Antibody

20-abx301115
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 0.1 mg
EUR 269

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

DNA Polymerase Lambda (POLL) Antibody (HRP)

20-abx317655
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody (FITC)

20-abx317656
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

DNA Polymerase Lambda (POLL) Antibody (Biotin)

20-abx317657
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Anti-DNA Polymerase lambda/POLL Antibody

A00959-2 100ug/vial
EUR 294

Polymerase (DNA directed), lambda (Recombinant)

20-abx073724
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 448

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 200ul
EUR 304

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 0.1 mg
EUR 269

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 0.5mg
EUR 435

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

Human DNA polymerase lambda, POLL ELISA KIT

ELI-08853h 96 Tests
EUR 824

Mouse DNA polymerase lambda, Poll ELISA KIT

ELI-47091m 96 Tests
EUR 865

Alpha Fluor™ 488 amine

1705 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine

1900 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

POLL Polymerase (DNA directed), Lambda Human Recombinant Protein

PROTQ9UGP5 Regular: 10ug
EUR 317
Description: POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.;POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Alpha Fluor™ 488 NHS Ester

1812 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

SV-A488-100 100 tests
EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 0.5 ml
EUR 225

DNA pol lambda Antibody

AF9055 200ul
EUR 304
Description: DNA pol λ Antibody detects endogenous levels of total DNA pol λ.

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 10 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

DNA Polymerase

ABF5235 100 ug
EUR 438

DNA Polymerase

ABD3082 100 ug
EUR 438

Anti-DNA pol lambda antibody

STJ92743 200 µl
EUR 197
Description: Rabbit polyclonal to DNA pol lambda.

Mouse IgG1-Alexa 488 conjugate (isotype control)

20102-101-A488 50 Tests
EUR 263

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.

DNA Polymerase beta Antibody

20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

DNA Polymerase beta Antibody

20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 438

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 390

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 252

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 187

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 252

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 187

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 252

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 187

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 252

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 187

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 333

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 239

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 252

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 187

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 390

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 100 tests
EUR 269

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

Lambda DNA (dam-, dcm-)

MDD21 100ug
EUR 104.81
  • Product category: PCR Related/Vectors/Plasmids

Lambda DNA (dam+, dcm+)

MDD22 500ug
EUR 214.43
  • Product category: PCR Related/Vectors/Plasmids

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Taq DNA Polymerase

BA00103 500U
EUR 87
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 145
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 239
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 522
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 123
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 196
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 413
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 145
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 239
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 522
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 120
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 190
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Kodaq DNA Polymerase

G498 250 U (50 ul)
EUR 87

Kodaq DNA Polymerase

G499 1000 U (200 ul)
EUR 158

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

PFU DNA Polymerase

9003-2500
EUR 512

PFU DNA Polymerase

9003-500
EUR 180

Laq? DNA Polymerase

9004-2500
EUR 610

Laq? DNA Polymerase

9004-500
EUR 218

DNA polymerase ? (Rat)

10-101 20ug
EUR 388
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 923
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

T4 DNA Polymerase

N101-01 2000 U
EUR 232

T4 DNA Polymerase

M1211-100
EUR 321

Phi29 DNA Polymerase

M1239-1000
EUR 311

Ready? DNA Polymerase

M1146-1000
EUR 332

Ready? DNA Polymerase

M1146-10000
EUR 1066

Ready? DNA Polymerase

M1146-5000
EUR 800

Breeze? DNA Polymerase

M1148-1000
EUR 1039

Breeze? DNA Polymerase

M1148-250
EUR 387

Distant? DNA Polymerase

M1150-1000
EUR 686

Distant? DNA Polymerase

M1150-250
EUR 278

Advance? DNA Polymerase

M1151-1000
EUR 746

Advance? DNA Polymerase

M1151-250
EUR 311

Outstretched? DNA Polymerase

M1152-1000
EUR 800

Outstretched? DNA Polymerase

M1152-250
EUR 332

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

AceTaq DNA Polymerase

P401-d1 250 U
EUR 136

AceTaq DNA Polymerase

P401-d2 1000 U
EUR 227

AceTaq DNA Polymerase

P401-d3 3000 U
EUR 447

Pfu DNA Polymerase

S116 1x250 units
EUR 76

Pfu DNA Polymerase

S117 2x250 units
EUR 94

Pfu DNA Polymerase

S118 10x250 units
EUR 303

Bst DNA Polymerase

S600 2000 U
EUR 107

Anti-Hu Ig Lambda Light Chain Alexa Fluor700

A7-673-T025 25 tests
EUR 154

Anti-Hu Ig Lambda Light Chain Alexa Fluor700

A7-673-T100 100 tests
EUR 269

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

S0005 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 200ul
EUR 304

DNA Polymerase beta Conjugated Antibody

C49605 100ul
EUR 397

DNA Polymerase β Conjugated Antibody

C33357 100ul
EUR 397

DNA Polymerase ζ Conjugated Antibody

C33602 100ul
EUR 397

DNA Polymerase θ Conjugated Antibody

C33611 100ul
EUR 397

DNA Polymerase α Conjugated Antibody

C33665 100ul
EUR 397

DNA Polymerase lamda Conjugated Antibody

C34098 100ul
EUR 397

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 505.25
  • Immunogen: polymerase(DNA directed), beta
  • Uniprot ID: P06746
  • Research Area: Metabolism
Description: Antibody raised against DNA polymerase beta

DNA Polymerase Kappa (POLK) Antibody

20-abx126383
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Kappa (POLK) Antibody

20-abx126384
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Kappa (POLK) Antibody

20-abx126385
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

DNA Polymerase Eta (POLH) Antibody

abx117166-100ug 100 ug
EUR 467
  • Shipped within 5-10 working days.

Polymerase (Dna Directed) iota Antibody

20-abx114586
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

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