Identification of novel hepatitis B virus therapeutic vaccine

Identification of novel hepatitis B virus therapeutic vaccine candidates derived from polymerase protein

Hepatitis B virus (HBV) an infection is a worldwide well being downside with excessive morbidity and mortality charges. The therapeutic vaccine is a promising methodology of remedy, and HBV polymerase performs an important position in viral replication. Due to this fact, a therapeutic vaccine that binds to HBV DNA polymerase might management HBV an infection. We predicted and chosen epitopes of polymerase utilizing on-line databases and evaluation software program. We then carried out molecular docking and peptide binding assays to judge the binding energies and affinities between polymerase epitopes and the HLA-A0201 molecule.

Lastly, we induced T cells from the peripheral blood mononuclear cells (PBMCs) of wholesome donors utilizing every epitope and quantified the capabilities of epitope-specific T cells by IFN-γELISPOT assay, T2 cell cytotoxicity assay, HepG2.2.15 cell cytotoxicity assay and HBV gene expression assays. 4 epitopes (RVTGGVFLV, GLLGFAAPF, LLDDEAGPL and YMDDVVLGA) had low binding power and two epitopes (RVTGGVFLV and GLLGFAAPF) had a excessive binding affinity. The T cells stimulated by two epitopes (GLLGFAAPF and HLYSHPIIL) had a higher means to induce immune response and suppress HBV. The HBV DNA polymerase epitopes recognized on this research are promising targets for designing an epitope-based therapeutic vaccine in opposition to HBV.

swggeo
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2×Taq PCR Master Mix(with dye)

K1034-5 5×1 ml
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2×Taq PCR Master Mix(with dye)

K1034-50 50×1 ml
EUR 514

2 × Taq Master Mix

P111-01 5 ml
EUR 117

2 × Taq Master Mix

P111-02 15 ml
EUR 146

2 × Taq Master Mix

P111-03 50 ml
EUR 236

Accuris Taq Master Mix

PR1001-1000 1 PC
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Accuris Taq Master Mix

PR1001-200 1 PC
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Accuris Taq Master Mix

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2 × Taq Plus Master Mix

P211-01 5 ml
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2 × Taq Plus Master Mix

P211-02 15 ml
EUR 193

2 × Taq Plus Master Mix

P211-03 50 ml
EUR 389

2 × Rapid Taq Master Mix

P222-01 5 ml (5×1ml)
EUR 120

2 × Rapid Taq Master Mix

P222-02 15 ml (15×1ml)
EUR 153

2 × Rapid Taq Master Mix

P222-03 50 ml (50 x 1 ml)
EUR 257

2 × Rapid Taq Master Mix

P222-04 50 ml (10×5ml)
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Accuris Taq Plus Master Mix

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2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn

W0655-1 NULL
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2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn

W0655-5 NULL
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2× EpiArt HS Taq Master Mix

EM201-01 1ml
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2× EpiArt HS Taq Master Mix

EM201-02 5 ml (5×1ml)
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2× EpiArt HS Taq Master Mix

EM201-03 15 ml (15×1ml)
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2 × Taq Master Mix (Dye Plus)

P112-01 5 ml
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2 × Taq Master Mix (Dye Plus)

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2 × Taq Plus Master Mix (Dye Plus)

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2 × Taq Plus Master Mix (Dye Plus)

P212-03 50 ml
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Taq PCR Master Mix (2X, Blue Dye)

BS9295 1ml
EUR 84.8
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Taq PCR Master Mix (2X, Blue Dye)

BS9296 5ml
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Hot Start Taq 2x Master Mix - 500 Reactions

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2× EpiArt HS Taq Master Mix (Dye Plus)

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2 × Taq Plus Master Mix II (Dye Plus)

P213-01 5 ml
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P213-02 15 ml
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Accuris Hot Start Taq Master Mix Red Dye

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Accuris Hot Start Taq Master Mix Red Dye

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Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
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Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 292.73
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Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
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Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
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Green Taq Mix

P131-01 5 ml
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P131-02 15 ml
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Jade? Master Mix

M1105-500
EUR 441

Taqman Master Mix

M1121-500
EUR 441

Fast Probe Master Mix with Rox (200 rxn)

31016 2x1mL
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31016-1 5x1mL
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31016-2 50x1mL
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2 × AceTaq Master Mix

P411-01 1 ml
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amfiSure PCR Master Mix

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amfiSure PCR Master Mix

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EUR 1873

Taqman Master Mix-iCycler

M1122-500
EUR 441

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M1124-500
EUR 441

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M1125-500
EUR 441

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 669

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 479.35
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Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2054.48
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9K-001-0005 250U
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EUR 2399
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SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

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2 × Phanta Max Master Mix

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2 × Phanta Max Master Mix

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EUR 575

amfiSure Prime PCR Master Mix

P1311-025 5X50 rxns
EUR 161

amfiSure Prime PCR Master Mix

P1311-050 10X50 rxns
EUR 219

amfiSure Prime PCR Master Mix

P1311-125 15X50 rxns
EUR 273

amfiSure Prime PCR Master Mix

P1311-250 50x50 rxns
EUR 739

amfiSure Prime PCR Master Mix

P1311-500 100x50 rxns
EUR 1238

amfiSure Advanced PCR Master Mix

P2311-025 5X50 rxns
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amfiSure Advanced PCR Master Mix

P2311-050 10X50 rxns
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amfiSure Advanced PCR Master Mix

P2311-125 15X50 rxns
EUR 282

amfiSure Advanced PCR Master Mix

P2311-250 50x50 rxns
EUR 752

amfiSure Advanced PCR Master Mix

P2311-500 100x50 rxns
EUR 1279

2 × Vazyme LAmp Master Mix

P311-01 1 ml
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2 × Vazyme LAmp Master Mix

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2 × Vazyme LAmp Master Mix

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EUR 401

Taqman Master Mix-Low ROX

M1123-500
EUR 441

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
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amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
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amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

AceQ qPCR Probe Master Mix

Q112-02 500 rxn (20 μl/rxn)
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AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
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AceQ U+ Probe Master Mix

Q113-02 500 rxn (20 μl/rxn)
EUR 267

AceQ U+ Probe Master Mix

Q113-03 2500 rxn (20 μl/rxn)
EUR 842

Selective Inhibition of DNA Polymerase β by a Covalent Inhibitor

DNA polymerase β (Pol β) performs an important position in DNA restore and has been intently linked to most cancers. Selective inhibitors of this enzyme are missing. Impressed by DNA lesions produced by antitumor brokers that inactivate Pol β, we’ve got undertaken the event of covalent small-molecule inhibitors of this enzyme. Utilizing a two-stage course of involving chemically synthesized libraries, we recognized a potent irreversible inhibitor (<b>14</b>) of Pol β (<i>Okay</i><sub>I</sub> = 1.8 ± 0.45 μM, <i>okay</i><sub>inact</sub> = (7.0 ± 1.0) × 10<sup>-3</sup> s<sup>-1</sup>). Inhibitor <b>14</b> selectively inactivates Pol β over different DNA polymerases. LC-MS/MS evaluation of trypsin digests of Pol β handled with <b>14</b> recognized two lysines inside the polymerase binding web site which might be covalently modified, one in all which was beforehand decided to play a task in DNA binding.

Fluorescence anisotropy experiments present that pretreatment of Pol β with <b>14</b> prevents DNA binding. Experiments utilizing a pro-inhibitor (<i>professional</i>-<b>14</b>) in wild kind mouse embryonic fibroblasts (MEFs) point out that the inhibitor (5 μM) is itself not cytotoxic however works synergistically with the DNA alkylating agent, methylmethanesulfonate (MMS), to kill cells.

Diagnostic efficiency of real-time polymerase chain response assay on blood for invasive aspergillosis and mucormycosis

Targets: This research aimed to judge the diagnostic usefulness of real-time (RT) polymerase chain response (PCR) on blood samples for prognosis of invasive aspergillosis and mucormycosis in sufferers with suspected invasive mould an infection.

Strategies: Grownup sufferers with suspected invasive mould an infection had been prospectively enrolled at a tertiary referral hospital in Seoul, South Korea between 2017 and 2020. Customary exams for prognosis of invasive mould an infection and RT-PCR for Aspergillus, Mucor, and Rhizopus utilizing blood samples had been carried out. We evaluated the diagnostic efficiency of RT-PCR exams in sufferers recognized with confirmed and possible invasive aspergillosis or mucormycosis an infection, in line with the modified definitions of the EORTC/MSG 2019.

Outcomes: A complete of 102 sufferers with suspected invasive mould an infection had been enrolled. Of those sufferers, 46 (45%) had been labeled as having confirmed (n=13) or possible (n=33) invasive aspergillosis, 21 (21%) as confirmed (n=17) or possible (n=4) invasive mucormycosis, and 18 (18%) as doable invasive mould an infection. The remaining 13 (13%) had been labeled as not having invasive mould an infection. Sufferers with doable invasive mould an infection (n=18) and coinfection of aspergillosis and mucormycosis (n=4) had been excluded from the ultimate evaluation. The sensitivity and specificity of the Aspergillus PCR had been 54.3% ([25/46], 95% confidence interval [CI]: 40.2-67.9%) and 94.1% ([32/34], 95% CI: 80.9-98.4%), respectively. The sensitivity and specificity of the Mucor or Rhizopus PCR had been 57.1% ([12/21], 95% CI: 36.6-75.5%) and 76.3% ([45/59], 95% CI: 64.0-85.3), respectively.

Conclusions: Our research means that blood PCR could be a helpful adjunct take a look at for diagnosing sufferers with suspected invasive mould an infection.

Facilitating SARS CoV-2 RNA-Dependent RNA polymerase (RdRp) drug discovery by assistance from HCV NS5B palm subdomain binders: In silico approaches and benchmarking

Corona Virus 2019 Illness (COVID-19) is a quickly rising pandemic attributable to a newly found beta coronavirus, known as Sever Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2). SARS CoV-2 is an enveloped, single stranded RNA virus that relies on RNA-dependent RNA polymerase (RdRp) to copy. Due to this fact, SARS CoV-2 RdRp is taken into account as a promising goal to stop virus replication. SARS CoV-2 polymerase exhibits excessive structural similarity to Hepatitis C Virus-1b genotype (HCV-1b) polymerase. Arising from the excessive similarity between SARS CoV-2 RdRp and HCV NS5B, we utilized the reported small-molecule binders to the palm subdomain of HCV NS5B (genotype 1b) to generate a high-high quality DEKOIS 2.Zero benchmark set and carried out a benchmarking evaluation in opposition to HCV NS5B.

The three extremely cited and publicly accessible docking instruments AutoDock Vina, FRED and PLANTS had been benchmarked. Primarily based on the benchmarking outcomes and evaluation by way of pROC-Chemotype plot, PLANTS confirmed the most effective screening efficiency and may acknowledge potent binders on the early enrichment. Accordingly, we used PLANTS in a potential digital screening to repurpose each the FDA-approved medicine (DrugBank) and the HCV-NS5B palm subdomain binders (BindingDB) for SARS CoV-2 RdRp palm subdomain.

Additional evaluation by molecular dynamics simulations for 50 ns really helpful diosmin (from DrugBank) and compound 3 (from BindingDB) to be the most effective potential binders to SARS CoV-2 RdRp palm subdomain. The perfect predicted compounds are really helpful to be biologically investigated in opposition to COVID-19. In conclusion, this work supplies in-silico evaluation to suggest doable SARS CoV-2 RdRp palm subdomain binders really helpful as a treatment for COVID-19. Up-to-our data, this research is the primary to suggest binders on the palm subdomain of SARS CoV2 RdRp. Moreover, this research delivers an instance of find out how to make use of a top quality custom-made DEKOIS 2.0 benchmark set as a process to raise the digital screening success fee in opposition to an important goal of the quickly rising pandemic.

DNA Polymerase Iota (POLI) Antibody
20-abx323859
  • EUR 314.00
  • EUR 244.00
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DNA Polymerase Iota (POLI) Antibody
20-abx333843
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
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  • 1 mg
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DNA Polymerase Iota (POLI) Antibody
abx236612-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.
Anti-DNA Polymerase iota (8G9)
YF-MA11349 100 ug
EUR 363
Description: Mouse monoclonal to DNA Polymerase iota
DNA polymerase iota (POLI) polyclonal antibody
ABP-PAB-11630 100 ug Ask for price
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DNA Polymerase Iota (POLI) Antibody (HRP)
20-abx335403
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DNA Polymerase Iota (POLI) Antibody (FITC)
20-abx335404
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DNA Polymerase Iota (POLI) Antibody (Biotin)
20-abx335405
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Anti-DNA Polymerase iota/POLI Antibody
A01376-1 100ug/vial
EUR 294
Donkey anti Goat IgG (H + L) (Alexa Fluor 594)
43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)
Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)
Donkey anti Rat IgG (H + L) (Alexa Fluor 594)
43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)
Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)
Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)
43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)
AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]
16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]
23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200
EGFR antibody (Alexa Fluor 488)
61R-E109BAF 125 ug
EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)
Endoglin/CD105 Alexa Fluor
FC15024 100 Tests
EUR 448
Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated
S0005 200ul
EUR 376
Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
S0006 200ul
EUR 376
Mouse DNA polymerase iota, Poli ELISA KIT
ELI-12742m 96 Tests
EUR 865
Human DNA polymerase iota, POLI ELISA KIT
ELI-38100h 96 Tests
EUR 824
Human DNA Polymerase Iota (POLI) ELISA Kit
abx382346-96tests 96 tests
EUR 911
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Anti-CD40 antibody (Alexa-fluor 488)
STJ170000 100 µg
EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin
Anti-LAMP3 antibody (Alexa-fluor 488)
STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.
Anti-LAMP3 antibody (Alexa-fluor 546)
STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.
Anti-LAMP3 antibody (Alexa-fluor 647)
STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.
Anti-IL3RA antibody (Alexa-fluor 488)
STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment
Anti-IL3RA antibody (Alexa-fluor 546)
STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment
Anti-IL3RA antibody (Alexa-fluor 647)
STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment
Anti-CD207 antibody (Alexa-fluor 488)
STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
Anti-CD207 antibody (Alexa-fluor 546)
STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
Anti-CD207 antibody (Alexa-fluor 647)
STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)
Anti-IL7R antibody (Alexa-fluor 488)
STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)
Anti-IL7R antibody (Alexa-fluor 546)
STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)
Anti-IL7R antibody (Alexa-fluor 647)
STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)
Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated
Ab8011-001 1mg
EUR 334
Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)
43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)
SAM FCM (Alexa Fluor 647)
abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.
SAM FCM (Alexa Fluor 488)
abx098904-60tests 60 tests
EUR 1358
  • Shipped within 5-10 working days.
Anti-RPSA Alexa Fluor® 488
A4-829-C100 0.1 mg
EUR 310
Anti-DNA pol iota Antibody
A01376 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.
Anti-DNA pol iota antibody
STJ92742 200 µl
EUR 197
Description: Rabbit polyclonal to DNA pol iota.
Anti-Hu CD16 Alexa Fluor® 488
A4-646-T100 100 tests
EUR 269
Goat anti Mouse IgG1 (Alexa Fluor 488)
43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)
Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)
50337 0.5 ml
EUR 225
Alpha Fluor™ 594 C5 Maleimide
1891 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
Streptavidin-Alexa594 (Alexas fluor 594) conjugate
SV-A594-100 100 tests
EUR 225
Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]
1795 10 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
DNA Polymerase
ABF5235 100 ug
EUR 438
DNA Polymerase
ABD3082 100 ug
EUR 438
DNA Polymerase alpha Antibody
AF5235 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.
DNA Polymerase beta Antibody
AF0148 200ul
EUR 304
Description: DNA Polymerase beta antibody detects endogenous levels of total DNA Polymerase beta.
DNA Polymerase beta Antibody
20-abx008852
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
DNA Polymerase beta Antibody
20-abx013060
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.
DNA Polymerase alpha Antibody
20-abx013375
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.
DNA Polymerase beta Antibody
ABF0148 100 ug
EUR 438
DNA polymerase beta Antibody
abx232432-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.
DNA Polymerase beta antibody
70R-50226 100 ul
EUR 244
Description: Purified Polyclonal DNA Polymerase beta antibody
DNA Polymerase zeta antibody
70R-33711 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody
DNA Polymerase zeta antibody
70R-33727 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase zeta antibody
DNA Polymerase theta antibody
70R-33737 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase theta antibody
DNA Polymerase beta antibody
70R-31161 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase beta antibody
DNA Polymerase alpha antibody
70R-31578 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase alpha antibody
DNA Polymerase lambda antibody
70R-32186 100 ug
EUR 327
Description: Rabbit polyclonal DNA Polymerase lambda antibody
DNA Polymerase beta Antibody
35404-100ul 100ul
EUR 390
DNA Polymerase λ Antibody
34098-100ul 100ul
EUR 252
DNA Polymerase λ Antibody
34098-50ul 50ul
EUR 187
DNA Polymerase ζ Antibody
33602-100ul 100ul
EUR 252
DNA Polymerase ζ Antibody
33602-50ul 50ul
EUR 187
DNA Polymerase θ Antibody
33611-100ul 100ul
EUR 252
DNA Polymerase θ Antibody
33611-50ul 50ul
EUR 187
DNA Polymerase α Antibody
33665-100ul 100ul
EUR 252
DNA Polymerase α Antibody
33665-50ul 50ul
EUR 187
DNA Polymerase beta Antibody
49605-100ul 100ul
EUR 333
DNA Polymerase beta Antibody
49605-50ul 50ul
EUR 239
DNA Polymerase β Antibody
33357-100ul 100ul
EUR 252
DNA Polymerase β Antibody
33357-50ul 50ul
EUR 187
DNA Polymerase gamma antibody
22870-100ul 100ul
EUR 390
DNA polymerase gamma antibody
70R-12532 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody
DNA Polymerase alpha Antibody
DF3082 200ul
EUR 304
Description: DNA Polymerase α Antibody detects endogenous levels of total DNA Polymerase α.
Anti-Hu CD72 Alexa Fluor® 488
A4-310-T100 100 tests
EUR 269
Anti-Bov CD9 Alexa Fluor® 488
A4-354-C100 0.1 mg
EUR 269
Anti-Hu CD30 Alexa Fluor® 700
A7-455-T100 100 tests
EUR 269
Anti-Hu CD94 Alexa Fluor® 700
A7-727-T100 100 tests
EUR 269
Anti-Hu CD56 Alexa Fluor® 700
A7-789-T100 100 tests
EUR 269
Donkey anti Goat IgG (H + L) (Alexa Fluor 647)
43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)
Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)
43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)
AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]
16890 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]
16891 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

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