Identification of novel hepatitis B virus therapeutic vaccine

Identification of novel hepatitis B virus therapeutic vaccine candidates derived from polymerase protein

Hepatitis B virus (HBV) an infection is a worldwide well being downside with excessive morbidity and mortality charges. The therapeutic vaccine is a promising methodology of remedy, and HBV polymerase performs an important position in viral replication. Due to this fact, a therapeutic vaccine that binds to HBV DNA polymerase might management HBV an infection. We predicted and chosen epitopes of polymerase utilizing on-line databases and evaluation software program. We then carried out molecular docking and peptide binding assays to judge the binding energies and affinities between polymerase epitopes and the HLA-A0201 molecule.

Lastly, we induced T cells from the peripheral blood mononuclear cells (PBMCs) of wholesome donors utilizing every epitope and quantified the capabilities of epitope-specific T cells by IFN-γELISPOT assay, T2 cell cytotoxicity assay, HepG2.2.15 cell cytotoxicity assay and HBV gene expression assays. 4 epitopes (RVTGGVFLV, GLLGFAAPF, LLDDEAGPL and YMDDVVLGA) had low binding power and two epitopes (RVTGGVFLV and GLLGFAAPF) had a excessive binding affinity. The T cells stimulated by two epitopes (GLLGFAAPF and HLYSHPIIL) had a higher means to induce immune response and suppress HBV. The HBV DNA polymerase epitopes recognized on this research are promising targets for designing an epitope-based therapeutic vaccine in opposition to HBV.

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2×Taq PCR Master Mix(with dye)

K1034-50 50×1 ml
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2 × Taq Plus Master Mix

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2 × Taq Plus Master Mix

P211-02 15 ml
EUR 231.6

2 × Taq Plus Master Mix

P211-03 50 ml
EUR 466.8

2 × Rapid Taq Master Mix

P222-01 5 ml (5×1ml)
EUR 144

2 × Rapid Taq Master Mix

P222-02 15 ml (15×1ml)
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2 × Rapid Taq Master Mix

P222-03 50 ml (50 x 1 ml)
EUR 308.4

2 × Rapid Taq Master Mix

P222-04 50 ml (10×5ml)
EUR 308.4

Accuris Taq Plus Master Mix

PR1001-TP-1000 1 PC
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Accuris Taq Plus Master Mix

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Accuris Taq Plus Master Mix

PR1001-TP-S 1 PC
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2× EpiArt HS Taq Master Mix

EM201-01 1ml
EUR 174

2× EpiArt HS Taq Master Mix

EM201-02 5 ml (5×1ml)
EUR 363.6

2× EpiArt HS Taq Master Mix

EM201-03 15 ml (15×1ml)
EUR 770.4

2 × Taq Master Mix (Dye Plus)

P112-01 5 ml
EUR 140.4

2 × Taq Master Mix (Dye Plus)

P112-02 15 ml
EUR 175.2

2 × Taq Master Mix (Dye Plus)

P112-03 50 ml
EUR 283.2

Accuris Hot Start Taq Master Mix

PR1001-HS-1000 1 PC
EUR 589.04

Accuris Hot Start Taq Master Mix

PR1001-HS-200 1 PC
EUR 203.36

Accuris Taq Master Mix Red Dye

PR1001-R-1000 1 PC
EUR 360.31

Accuris Taq Master Mix Red Dye

PR1001-R-200 1 PC
EUR 145.94

Accuris Taq Master Mix Red Dye

PR1001-R-S 1 PC
EUR 83.75

Taq PCR Master Mix (2X, Blue Dye)

BS9295 1ml
EUR 101.76

Taq PCR Master Mix (2X, Blue Dye)

BS9296 5ml
EUR 227.04

Taq PCR Master Mix (2X, Red Dye)

BS9297 1ml
EUR 101.76

Taq PCR Master Mix (2X, Red Dye)

BS9298 5ml
EUR 227.04

2 × Taq Plus Master Mix (Dye Plus)

P212-01 5 ml
EUR 159.6

2 × Taq Plus Master Mix (Dye Plus)

P212-02 15 ml
EUR 231.6

2 × Taq Plus Master Mix (Dye Plus)

P212-03 50 ml
EUR 466.8

2× EpiArt HS Taq Master Mix (Dye Plus)

EM202-01 1ml
EUR 174

2× EpiArt HS Taq Master Mix (Dye Plus)

EM202-02 5 ml (5×1ml)
EUR 363.6

2× EpiArt HS Taq Master Mix (Dye Plus)

EM202-03 15 ml (15×1ml)
EUR 770.4

2 × Taq Plus Master Mix II (Dye Plus)

P213-01 5 ml
EUR 160.8

2 × Taq Plus Master Mix II (Dye Plus)

P213-02 15 ml
EUR 231.6

2 × Taq Plus Master Mix II (Dye Plus)

P213-03 50 ml
EUR 466.8

Accuris Hot Start Taq Master Mix Red Dye

PR1001-HSR-1000 1 PC
EUR 589.04

Accuris Hot Start Taq Master Mix Red Dye

PR1001-HSR-200 1 PC
EUR 203.36

Accuris Hot Start Taq Master Mix Red Dye

PR1001-HSR-S 1 PC
EUR 83.75

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Green Taq Mix

P131-01 5 ml
EUR 142.8

Green Taq Mix

P131-02 15 ml
EUR 180

Green Taq Mix

P131-03 50 ml
EUR 297.6

Jade? Master Mix

M1105-500 each
EUR 529.2

Taqman Master Mix

M1121-500 each
EUR 529.2

Fast Probe Master Mix with Rox (200 rxn)

31016 2x1mL
EUR 280.8
Description: Minimum order quantity: 1 unit of 2x1mL

Fast Probe Master Mix with Rox (500 rxn)

31016-1 5x1mL
EUR 559.2
Description: Minimum order quantity: 1 unit of 5x1mL

Fast Probe Master Mix with Rox (5000 rxn)

31016-2 50x1mL
EUR 4645.2
Description: Minimum order quantity: 1 unit of 50x1mL

amfiSure PCR Master Mix

P0311-010 2x50 rxns
EUR 151.2

amfiSure PCR Master Mix

P0311-025 5X50 rxns
EUR 198

amfiSure PCR Master Mix

P0311-050 10X50 rxns
EUR 262.8

amfiSure PCR Master Mix

P0311-100 10x1ml
EUR 343.2

amfiSure PCR Master Mix

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EUR 333.6

amfiSure PCR Master Mix

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EUR 562.8

amfiSure PCR Master Mix

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EUR 902.4

amfiSure PCR Master Mix

P0311-500 100x50 rxns
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amfiXpand PCR Master Mix

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amfiXpand PCR Master Mix

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amfiXpand PCR Master Mix

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2 × AceTaq Master Mix

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M1122-500 each
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Taq DNA Polymerase - 5,000 units with separate dNTP Mix

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EUR 802.8

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

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amfiSure Prime PCR Master Mix

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amfiSure Prime PCR Master Mix

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amfiSure Advanced PCR Master Mix

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amfiSure Advanced PCR Master Mix

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amfiSure Advanced PCR Master Mix

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2 × Phanta Max Master Mix

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SYBR Green qPCR Master Mix

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Fast Probe Master Mix with Rox, trial size (100 rxn)

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EUR 130.8
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Description: Minimum order quantity: 1 unit of 2x1mL

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Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)

31014-2 50x1mL
EUR 4291.2
Description: Minimum order quantity: 1 unit of 50x1mL

Selective Inhibition of DNA Polymerase β by a Covalent Inhibitor

DNA polymerase β (Pol β) performs an important position in DNA restore and has been intently linked to most cancers. Selective inhibitors of this enzyme are missing. Impressed by DNA lesions produced by antitumor brokers that inactivate Pol β, we’ve got undertaken the event of covalent small-molecule inhibitors of this enzyme. Utilizing a two-stage course of involving chemically synthesized libraries, we recognized a potent irreversible inhibitor (<b>14</b>) of Pol β (<i>Okay</i><sub>I</sub> = 1.8 ± 0.45 μM, <i>okay</i><sub>inact</sub> = (7.0 ± 1.0) × 10<sup>-3</sup> s<sup>-1</sup>). Inhibitor <b>14</b> selectively inactivates Pol β over different DNA polymerases. LC-MS/MS evaluation of trypsin digests of Pol β handled with <b>14</b> recognized two lysines inside the polymerase binding web site which might be covalently modified, one in all which was beforehand decided to play a task in DNA binding.

Fluorescence anisotropy experiments present that pretreatment of Pol β with <b>14</b> prevents DNA binding. Experiments utilizing a pro-inhibitor (<i>professional</i>-<b>14</b>) in wild kind mouse embryonic fibroblasts (MEFs) point out that the inhibitor (5 μM) is itself not cytotoxic however works synergistically with the DNA alkylating agent, methylmethanesulfonate (MMS), to kill cells.

Diagnostic efficiency of real-time polymerase chain response assay on blood for invasive aspergillosis and mucormycosis

Targets: This research aimed to judge the diagnostic usefulness of real-time (RT) polymerase chain response (PCR) on blood samples for prognosis of invasive aspergillosis and mucormycosis in sufferers with suspected invasive mould an infection.

Strategies: Grownup sufferers with suspected invasive mould an infection had been prospectively enrolled at a tertiary referral hospital in Seoul, South Korea between 2017 and 2020. Customary exams for prognosis of invasive mould an infection and RT-PCR for Aspergillus, Mucor, and Rhizopus utilizing blood samples had been carried out. We evaluated the diagnostic efficiency of RT-PCR exams in sufferers recognized with confirmed and possible invasive aspergillosis or mucormycosis an infection, in line with the modified definitions of the EORTC/MSG 2019.

Outcomes: A complete of 102 sufferers with suspected invasive mould an infection had been enrolled. Of those sufferers, 46 (45%) had been labeled as having confirmed (n=13) or possible (n=33) invasive aspergillosis, 21 (21%) as confirmed (n=17) or possible (n=4) invasive mucormycosis, and 18 (18%) as doable invasive mould an infection. The remaining 13 (13%) had been labeled as not having invasive mould an infection. Sufferers with doable invasive mould an infection (n=18) and coinfection of aspergillosis and mucormycosis (n=4) had been excluded from the ultimate evaluation. The sensitivity and specificity of the Aspergillus PCR had been 54.3% ([25/46], 95% confidence interval [CI]: 40.2-67.9%) and 94.1% ([32/34], 95% CI: 80.9-98.4%), respectively. The sensitivity and specificity of the Mucor or Rhizopus PCR had been 57.1% ([12/21], 95% CI: 36.6-75.5%) and 76.3% ([45/59], 95% CI: 64.0-85.3), respectively.

Conclusions: Our research means that blood PCR could be a helpful adjunct take a look at for diagnosing sufferers with suspected invasive mould an infection.

Facilitating SARS CoV-2 RNA-Dependent RNA polymerase (RdRp) drug discovery by assistance from HCV NS5B palm subdomain binders: In silico approaches and benchmarking

Corona Virus 2019 Illness (COVID-19) is a quickly rising pandemic attributable to a newly found beta coronavirus, known as Sever Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2). SARS CoV-2 is an enveloped, single stranded RNA virus that relies on RNA-dependent RNA polymerase (RdRp) to copy. Due to this fact, SARS CoV-2 RdRp is taken into account as a promising goal to stop virus replication. SARS CoV-2 polymerase exhibits excessive structural similarity to Hepatitis C Virus-1b genotype (HCV-1b) polymerase. Arising from the excessive similarity between SARS CoV-2 RdRp and HCV NS5B, we utilized the reported small-molecule binders to the palm subdomain of HCV NS5B (genotype 1b) to generate a high-high quality DEKOIS 2.Zero benchmark set and carried out a benchmarking evaluation in opposition to HCV NS5B.

The three extremely cited and publicly accessible docking instruments AutoDock Vina, FRED and PLANTS had been benchmarked. Primarily based on the benchmarking outcomes and evaluation by way of pROC-Chemotype plot, PLANTS confirmed the most effective screening efficiency and may acknowledge potent binders on the early enrichment. Accordingly, we used PLANTS in a potential digital screening to repurpose each the FDA-approved medicine (DrugBank) and the HCV-NS5B palm subdomain binders (BindingDB) for SARS CoV-2 RdRp palm subdomain.

Additional evaluation by molecular dynamics simulations for 50 ns really helpful diosmin (from DrugBank) and compound 3 (from BindingDB) to be the most effective potential binders to SARS CoV-2 RdRp palm subdomain. The perfect predicted compounds are really helpful to be biologically investigated in opposition to COVID-19. In conclusion, this work supplies in-silico evaluation to suggest doable SARS CoV-2 RdRp palm subdomain binders really helpful as a treatment for COVID-19. Up-to-our data, this research is the primary to suggest binders on the palm subdomain of SARS CoV2 RdRp. Moreover, this research delivers an instance of find out how to make use of a top quality custom-made DEKOIS 2.0 benchmark set as a process to raise the digital screening success fee in opposition to an important goal of the quickly rising pandemic.

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DNA Polymerase Iota (POLI) Antibody (FITC)

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DNA Polymerase Iota (POLI) Antibody (Biotin)

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Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

Human DNA Polymerase Iota (POLI) ELISA Kit

abx382346-96tests 96 tests
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Human DNA polymerase iota, POLI ELISA KIT

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Mouse DNA polymerase iota, Poli ELISA KIT

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Anti-DNA pol iota Antibody

A01376 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DNA pol iota Antibody (POLI) detection. Tested with WB in Human.

Anti-DNA pol iota antibody

STJ92742 200 µl
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Description: Rabbit polyclonal to DNA pol iota.

iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*

45035 100 Slides
EUR 334

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
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Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 405.6
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Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 436.8
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Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

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EUR 554.4
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Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

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EUR 411.6
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Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF 1 mg
EUR 337.2
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

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AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

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DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552 200ul
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DNA Polymerase theta Antibody

DF13563 100ul
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DNA Polymerase alpha Antibody

AF5235 200ul
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DNA Polymerase beta Antibody

AF0148 200ul
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DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
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DNA Polymerase zeta antibody

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DNA Polymerase theta antibody

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DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
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DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
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DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
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DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase gamma antibody

22870-100ul 100ul
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DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
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DNA Polymerase θ Antibody

33611-50ul 50ul
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DNA Polymerase α Antibody

33665-100ul 100ul
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DNA Polymerase α Antibody

33665-50ul 50ul
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35404-100ul 100ul
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DNA Polymerase λ Antibody

34098-100ul 100ul
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EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
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DNA Polymerase β Antibody

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EUR 224.4

DNA Polymerase beta Antibody

20-abx013060
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DNA Polymerase alpha Antibody

20-abx013375
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DNA Polymerase beta Antibody

20-abx008852
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DNA Polymerase beta Antibody

ABF0148 100 ug
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DNA polymerase beta Antibody

abx232432-100ug 100 ug
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Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
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Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

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IMMOLASE DNA Polymerase

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ACCUZYME DNA Polymerase

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ACCUZYME DNA Polymerase

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BIOTAQ DNA Polymerase

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MangoTaq DNA Polymerase

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MangoTaq DNA Polymerase

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MangoTaq DNA Polymerase

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VELOCITY DNA Polymerase

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MyTaq DNA Polymerase

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MyFi DNA Polymerase

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